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We assayed for phosphoserine phosphatase, enterobactin esterase, threonine deaminase, and citrate synthase activities in lysates from cells expressing the respective de novo proteins. The disadvantage of lysates, however, is that background activities may obscure the low-level activities of the de novo proteins. This was not surprising, particularly for the Fes and SerB activities, since previous work in our laboratory showed that E. Armirovanie Lysates are easy to prepare and are more likely to contain molecular partners (proteins, cofactors, or metals) present in vivo. In several cases, activity was observed, however, lysates from control cells also showed low levels of activity.